In vitro Doses and Incubations Dependent Thrombolytic Potential Study of Edible Mushrooms Pleurotus ostreatus, Ganoderma lucidum and Lentinula edodes Available in Bangladesh

Aims: The study was carried out to investigate the thrombolytic potential of methanolic extracts of edible mushrooms.
Study Design: Experimental study.
Place and Duration of Study: Department of Biochemistry, Primeasia University, August 2014 to December 2014
Methodology: An in vitro thrombolytic model was used to check the thrombolytic effect of Pleurotus ostreatus, Ganoderma lucidum and Lentinula edodes along with Streptokinase as a positive control and water as a negative control. In this study two concentrations of the extracts i.e. 400µg/ml and 800 µg/ml were tested at three time intervals, 24 hrs, 48 hrs and 72 hrs duration of incubation at 37ºC for observing maximum clot lysis.
Results: At 400 µg/ml dose, Pleurotus ostreatus showed highest clot lysis activity in 72 hrs of incubation, 18.62% while Ganoderma lucidum, and Lentinula edodes showed 17.01% and 9.02% in the same incubation. Similarly, at 800 µg/ml dose, the thrombolytic activities were 63.35%, 34.16%, and 29.00% respectively for Pleurotus ostreatus, Ganoderma lucidum, and Lentinula edodes. Our study clearly demonstrated that Pleurotus ostreatus exhibits maximum 63.37% clot lysis at 800 µg/ml concentration in 72 hrs of incubation with reference to Streptokinase 86.43%. The thrombolytic nature of the mushroom extracts was found to be significant (P<0.0001), when compared with the negative control (water) at different doses. The relative thrombolytic activity of Pleurotus ostreatus, Ganoderma lucidum and Lentinula edodes was found to significant at different doses (P<0.0001) and at various incubation periods (P<0.0001).
Conclusion: The result of our findings indicated that percentage of clot lysis is directly proportional to the concentration and incubation time of the extracts.