Examination of Conditions for Optimized Decellularized Liver Preparation

Aims: The main aim of our study was to examine the concentration of surfactant that can cause significant disruption of the resulting decellularized liver structure. Furthermore, it is our goal to determine the suitable solvent that can boost the potential of each surfactant.

Methodology: The porcine liver discs of 8-mm diameter and 2-mm thickness were prepared. These were soaked in aqueous solution of either sodium dodecyl sulfate (SDS) or Triton X-100 (TX), and placed on a rotational shaking machine (100 rpm).

Results: TX was unable to completely remove the cellular components under any of our experimental conditions. The salt concentration did not affect the decellularization in TX. The pH buffer, however, was found to affect the decellularization. Also, in the solvent study, the conditions under which SDS effectively exerted power were not the salt concentration and pH, but the condition that was close to water. We also confirmed that the shrinkage of tissue occurred when decellularization with 0.1% SDS in CMF-PBS. However, 0.1% SDS in distilled water didn't cause the deformation of tissue. This is considered to be due to the low salt concentration of solvent.

Conclusion: This work establishes the concentration range of the surfactant that causes the collapse of the cellular structure during decellularization. In addition, the solvent suitable for each surfactant has also been established.