Detection of TEM and SHV Genes in Clinical Escherichia coli and Klebsiella pneumoniae Strains ESBL Isolated in Neonatology and Pediatric Units

Gbonon, V and Afran, S and Guessennd, K and Toty, A and Diplo, T and N’Guetta, A and Dosso, M (2018) Detection of TEM and SHV Genes in Clinical Escherichia coli and Klebsiella pneumoniae Strains ESBL Isolated in Neonatology and Pediatric Units. Microbiology Research Journal International, 23 (2). pp. 1-7. ISSN 24567043

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Official URL: https://doi.org/10.9734/MRJI/2018/39354

Abstract

Aims: To detect blaTEM and blaSHV genes in Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) strains resistant to β-lactams isolated from neonatal and pediatric infections.

Place and Duration: Bacteriology-Virology Department and Molecular Biology platform of Pasteur Institute of Côte d'Ivoire from January 2012 to November 2015.

Methods: A total of 38 strains of E. coli and K. pneumoniae ESBL isolated and identified according to classical bacteriology techniques from neonatal and pediatric infections, were subject of our study. Search for blaTEM, and blaSHV genes were carried out by conventional PCR.

Results: Molecular research showed that 52.6% of strains were carrying blaTEM gene and blaSHV gene was present in 36.9%. blaTEM and blaSHV genes were present simultaneously in 36.9% of strains.

Conclusion: This study revealed a predominance of blaTEM genes in E. coli and K. pneumoniae ESBL strains.

Item Type:	Article
Subjects:	Grantha Library > Biological Science
Depositing User:	Unnamed user with email support@granthalibrary.com
Date Deposited:	09 May 2023 09:20
Last Modified:	20 Jun 2024 13:25
URI:	http://asian.universityeprint.com/id/eprint/712

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